Claudio Sette

Research Focus

My laboratory has been interested for many years in the study of intracellular signal transduction pathways and on their impact on gene expression at multiple levels. In particular, we are interested in cell differentiation processes that play a role in development and disease. In the last five years, we have focused our attention on the role played by Sam68 in these processes. Sam68 is a prototype of the STAR (Signal Transduction and Activation of RNA) family of RNA binding proteins. Sam68 is known to integrate signal transduction pathways with modulation of RNA processing and utilization, at the level of alternative splicing, nuclear export and cytoplasmic utilization.

Publications

1. Paronetto, M.P., Cappellari, M., Busà, R., Pedrotti, S., Vitali, R., Comstock, C., Hyslop, T., Knudsen, K.E., Sette, C. (2010). Alternative splicing of the proto-oncogene CCND1 is regulated by Sam68 in human prostate cancer. Cancer Research (in press).
2. Brignatz, C., Paronetto, M.P., Opi, S., Cappellari, M., Audebert, S., Feuillet, V., Bismuth, G., Roche, S., Arold, S.T., Sette, C., Collette, Y. (2009). Alternative splicing modulates auto-inhibition in the Src kinase Fyn. Molecular and Cellular Biology 29, 6438-6448.
3. Sette, C., Messina, V., Paronetto, M.P. (2009). Sam68: a new STAR in the fertility firmament. Journal of Andrology [Epub ahead of print]. (doi:10.2164/jandrol.109.008136)
4. Paronetto, M.P., Messina, V., Bianchi, E., Barchi, M., Vogel, G., Moretti, C., Palombi, F., Stefanini, M., Geremia, R., Richard, S., Sette, C. (2009). Sam68 regulates translation of target mRNAs in male germ cells, necessary for mouse spermatogenesis. Journal of Cell Biology 185, 235-49.
5. Paronetto, M.P., Sette, C. (2009). Role of RNA-binding proteins in mammalian spermatogenesis. International Journal of Andrology. Mar 5. [Epub ahead of print].
6. Bianchini, A., Loiarro, M., Bielli, P., Busà, R., Paronetto, M.P., Loreni, F., Geremia, R., Sette, C. (2008). Phosphorylation of eIF4E by MNKs supports protein synthesis, cell cycle progression and proliferation in prostate cancer cells. Carcinogenesis 29, 2279-88.
7. Ehrmann, I., Dalgliesh, C., Tsaousi, A., Paronetto, M.P., Heinrich, B., Kist, R., Cairns, P., Li, W., Mueller, C., Jackson, M., Peters, H., Nayernia, K., Saunders, P., Mitchell, M., Stamm, S., Sette, C., Elliott, D.J. (2008). Haploinsufficiency of the germ cell-specific nuclear RNA binding protein hnRNP G-T prevents functional spermatogenesis in the mouse. Human Molecular Genetics 17, 2803-18.
8. Paronetto, M.P., Bianchi, E., Geremia, R., Sette, C. (2008). Dynamic expression of the RNA-binding protein Sam68 during mouse pre-implantation development. Gene Expression Patterns 8, 311-22 .
9. Iwasaki, T., Koretomo, Y., Fukuda, T., Paronetto, M.P., Sette, C., Fukami, Y., Sato, K. (2008). Expression, phosphorylation, and mRNA-binding of heterogeneous nuclear ribonucleoprotein K in Xenopus oocytes, eggs, and early embryos. Development, Growth & Differentiation 50, 23-40.
10. Paronetto, M.P., Achsel, T., Massiello, A., Chalfant, C.E., and Sette, C. (2007). The RNA-binding protein Sam68 modulates the alternative splicing of Bcl-x. Journal of Cell Biology 176, 929-39.

Key lab techniques: study of signal transduction and analysis of animal models of defects in the regulation of RNA metabolism.

Lab contact: claudio.sette@uniroma2.it